In spite of abnormal meiotic products reducing pollen fertility, seed production was normal. Quadrivalents were observed in diakinesis at low frequency, which suggests a segmental allotetraploid and the inability of both genomes to co-ordinate their activities, leading to multiple spindle and precocious cellularization. The result consisted of a high frequency of abnormal products of meiosis. Some curious planes of cytokinesis were found in some cells, with partitioning of cytoplasm into cells of irregular shape. In several microsporocytes, the micronuclei with their minispindle were divided further into microcytes by additional cytokinesis. While in other polyploid accessions of Brachiaria micronuclei remained in this condition until the second cytokinesis, the micronuclei in this accession organized their own spindle in the second division. Due to its tetraploid condition, the accession presented many cells with precocious chromosome migration to the poles in metaphase I and laggards in anaphase I that gave rise to micronuclei in telophase I. In telophase I, the interzonal microtubules continued to be intensely stained, and during the phragmoplast formation the fibers were pushed to the cell wall, persisting until prophase II, even after cytokinesis. The spindle in metaphase I and anaphase I became heavily stained with propionic carmine. Basilisk, revealed an unusual pattern of microsporogenesis. ![]() Cytogenetic studies carried out in the tetraploid accession BRA001068 of Brachiaria decumbens, also known as cv.
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